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Sample |
Laser Capture Microdissection of pre-cancerous or invasive cells from matrix
tissues. Formalin-Fixed Tissue proteomic analysis HUPO standards for serum and plasma collection |
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Sample |
Trace Analysis: Lysis, digestion, labeling, and LC-MS proteomic analysis of less than 10,000 cells Multiple Lectin (MLAC) Chromatography to extract and fractionate glycoproteins from sera. Multidimensional Separations using IEF or SCX prior to RPLC-MS analysis. Tagging of proteins to enhance sensitivity (electrophores, Alexa-fluor) or to select protein classes of interest (ICAT, iTRAQ, 16O/18O differential labeling). |
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LC and |
ERPA (Extended Range Proteomic Analysis) 95% sequence coverage of large (180 kDa) proteins, with characterization of phosphorylation and glycosylation. 14 mass spectrometers, including LTQ-FTMS and a 2 kHz MALDI instrument constructed in-house. Ultranarrow Monolithic Capillary Columns: ultratrace analysis. Capillary LC and 2D LC (Advion NanoMate; LC Packings; Dionex) NanoSplitter: 1000-fold higher sensitivity in 4 mm LC-MS. |
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Data Processing |
MEND: denoising using information from LC dimension as well as MS dimension. PRESEL: optimized selection of precursor ions increases identifications by 30%. Linux cluster. |
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| Additional Capabilities | Bioorganic chemistry: immunotoxins; PSA-activated toxins or imaging agents. NMR: ultratrace microcoil NMR, LC-NMR. |
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| Applications | Biomarker discovery and validation: breast and cervical cancer, cardiovascular disease; DNA Adducts |
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