Comprehensive Protein Characterization: 

Extended Range Proteomic Analysis (ERPA) Detects Phosphorylation, Glycosylation, and Splicing Isoforms

 Overview by Shiaw-Lin Wu; Full Paper (J. Proteome Research  ASAP Article)

• Nano-LC/MS Method Using a Hybrid Ion Trap + Fourier Transform Mass Spectrometer.
• Coupling of FTICR survey scan with concurrent MS² and MS³ CID fragmentation.
• Larger fragments analyzed (to 10 kDa); can correlate PTM’s.
• 95% sequence coverage, with identification of posttranslational modifications, in EGFR (180 kDa).
• Characterized 6 fully glycosylated sites, 2 non-glycosylated, 4 partially glycosylated.
• 400 fmol sensitivity