Research
Activities:
- NMR Applications to
natural product identification and metabolite discovery.
-
Microcoil NMR for trace analysis of mass-limited samples.
-
Segmented-Flow applications in microfluidic devices.
-
LC-MS NMR methods.
Nuclear Magnetic
Resonance (NMR) combined with mass spectrometry is the general starting
point for
structural elucidation of unknown compounds. However, where thorough mass
spectrometric analysis typically takes under a second with a nanogram of
analyte, NMR requires hours to days at the microgram level.
Microcoil NMR probes, with volumes from 10 uL down to 30 nL have 10 - 50
times the mass sensitivity of a conventional 5 mm probe. The institute's
focus on microseparations generates demand for microscale NMR
methods. Several approaches to loading submicroliter samples through
flow systems with large dead volumes have been developed, allowing
efficient coupling of micro-NMR with microseparations.
Education:
Reed College, Portland OR BA 1976-1980 Physics
Stanford University, Palo Alto, CA Ph.D. 1985-1990 Biophysics
Yale University, New Haven, CT (postdoctoral) 1990-1992 Mol. Biophys &
Biochem
Massachusetts Institute of Technology (postdoctoral) 1993-1995 Chemistry
Selected
Publications, Patents, and Invited Presentations
Lin Y, Schiavo S, Orjala J, Vouros P, Kautz R. "Microscale
LC-MS-NMR platform applied to the identification of active cyanobacterial
metabolites". Anal Chem. 2008 80(21):8045-8054. (pubmed)
Kautz, RA “An Optimized
LC-MS+NMR Strategy Using a Microcoil NMR Probe with Normal-bore LC”.
Invited presentation, NIH/NIA Baltimore MD, 23 April 2008.
Microplug NMR: Increasing microcoil NMR sensitivity
using segmented flow sample loading, or “Shigemi tubes for microcoil
probes” Yiqing Lin and Roger Kautz* (poster,
at Advances in Metabolic Profiling, Boston, Nov 2007)
An Ultrasensitive LC-MS+NMR Platform Utilizing
Segmented-Flow Microcoil NMR, Nanospray ESI, and 4 mm LC
Columns, Yiqing Lin, Paul Vouros, Jimmy Orjala1, Roger Kautz*
(poster, at
Advances in Metabolic Profiling, Boston, Nov 2007)
An Offline LC-MS-microcoil
NMR System with High Sensitivity for Rapid Structure Elucidation of Small
Molecules from Complex Mixtures, Yiqing Lin, Jimmy Orjala,
Paul Vouros, Roger Kautz, invited oral presentation at the 55'th American
Society for Mass Spectrometry, June 5 2007, Indianapolis, IN.
Featured in Analytical Chemistry as "LC/MS and NMR are
Together at Last" J. Griffiths, (Sept 1 2007, p. 6431)
Lin
Y, Vouros P, Orjala J, and Kautz RA. “An Ultrasensitive LC-MS-NMR
Platform Utilizing Segmented-Flow Microcoil NMR, Nanospray ESI, and 4 mm
LC Columns. “ invited lecture, 48’th Experimental NMR
Conference, Daytona FL, 26 April 2007.
Gang
Shao, Roger Kautz, Shiqi Peng, Guohui Cui, and Roger W. Giese.
“Calibration by NMR for quantitative analysis: p-Toluenesulfonic Acid as
an Internal Standard” J. Chromatogr. A 1138(1-2): 305-308 (2007)
Kautz,
R.A., Karger, B.L. “Method of Efficient Transport of Small Liquid
Volumes To, From, or Within Microfluidic Devices” Patent
WO/2005/059512, PCT/US2004/041923.
Kautz,
R.A. , Goetzinger, W., and Karger, B.L. "High-Throughput
Microcoil NMR of Compound Libraries Using Zero-Dispersion Segmented Flow
Analysis". J. Combinatorial Chemistry, 7: 14-20 (2005).
(Among 10
most-accessed papers of this journal volume)
Kautz,
R.A. and Karger B.L. “Method for Efficient Transport of Small
Liquid Volumes To, From, or Within Microfluidic Devices”. U.S.
Patent Application Appl. 2004; Provisional No. 60/528,410, Dec 10,
2003;
Invited
Lecture, SMASH small molecule NMR conference, Varian Users Meeting,
Breckenridge CO, 2002
Kautz,
R.A., Lacey, M.E., Wolters, A.M., Foret, F., Webb, A.G., Karger, B.L. and
Sweedler, J.V. Sample Concentration and Separation for Nanoliter-Volume
NMR Spectroscopy Using Capillary Isotachophoresis J. Am. Chem.
Soc. 123(13): 3159-3160. (2001)
Hafner,
F.T., Kautz, R.A., Iverson, B.L., Tim, R.C. and Karger B.L.
Noncompetitive Immunoassay of Small Analytes at the Femtomolar Level by
Affinity Probe Capillary Electrophoresis: Direct Analysis of Digoxin Using
a Uniform-Labeled scFv Immunoreagent. Analytical Chemistry
72:5779-5786. (2000)
Tim,
R.C., Kautz, R.A., Karger, B.L. Ultratrace Analysis of Drugs in
Biological Fluids Using Affinity Probe Capillary Electrophoresis: Analysis
of Dorzolamide with Fluorescently Labeled Carbonic Anhydrase.
Electrophoresis 21:220-226 (2000)
Kautz,
R.A. and Karger, B.L. “Method for Noncompetitive Immunoassay Using
Affinity Probe Electrophoresis with Shift Ligand and Immunoaffinity
Extraction”, U.S. Provisional Patent Application, (1999)
Southworth,
M.W., Adam, M., Panne, D., Byer, R., Kautz, R.A, and Perler, F.B.
Control of Protein Splicing by Intein Fragment Reassembly. EMBO
Journal 17:918-926 (1998).
Kautz,
R.A., and Fox, R.O. NMR Analysis of Staphylococcal Nuclease Mutants
Thermal Quench Refolding Kinetics. Protein Science 2: 851-858
(1993).
Evans,
PA., Dobson, C.M., Kautz, R.A., Hatfull, G. and Fox, R.O. Proline
Isomerism In Staphylococcal Nuclease Characterized by NMR and
Site-Directed Mutagenesis. Nature 239:266-268 (1987).
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