Dr. William
S. Hancock
Raymond Bradford Bradstreet Chair
in Analytical Chemistry
|
| |
|
Education
1970 Ph.D., Adelaide University,
South Australia
1966 B.Sc., Adelaide University,
South Australia
e-mail:
wi.hancock@neu.edu
tel. 617-373-4881
FAX: 617-373-2855
Research
Interests
Analytical
and Protein Chemistry
Dr.
Hancock's research program is developing new analytical platforms
combined with new bioinformatics approaches, for applying
proteomics to the study of plasma and serum in disease.
A focus of this program is clinical proteomics, the search
for biomarkers that predict the progress of disease and can
be used to segregate patients for individualized therapy.
Several key advances have been incorporated. The
research group has developed a new sample pre-fractionation
approach, multi-lectin affinity chromatography (M-LAC), which
has successfully been used to detect known (BRCA1) and new
rare markers in sera of breast cancer patients. In
addition the M-LAC platform allows the deep study of changes
in the glycoproteome in plasma and we have demonstrated that
half of the proteins in blood are glycosylated. Another new
platform developed in the laboratory allows the study of disease
related proteolysis, named the "fragmentome", and
monitors the degradation of disease associated markers.
Dr.
Hancock's Research Group
|
|
| Selected
Publications (Complete List of Publications) |
| |
| Hancock,
W.S. and Hincapie, M., "A Two Step Fractionation
Approach for Plasma Proteomics Using Immunodepletion
of Abundant Proteins and Multi-lectin Affinity
Chromatography (M-LAC) : Application to the
Analysis of Obesity, Diabetes and Hypertension,
Dayarathna, MKD", J. Sep. Science,
(2008), in press. |
| |
| Orazine,
C.I., Hincapie, M., Hancock, W.S., Hattersley,
M., and Hanke, J., "A Proteomic Analysis
of the Plasma Glycoproteins of a MCF-7 Mouse
Xenograft : A Model System for the Detection
of Tumor Markers", J.Prot. Res.,
(2008), in press. |
| |
|
Lu, C., Murugesan, N., Macdonald, J. A., Wu,
S-L., Pachter, J., and Hancock, W. S., "Analysis
of Mouse Brain Microvascular Endothelium Using
Immuno-Laser Capture Microdissection Coupled
to a Hybrid LTQ-FT MS Proteomics Platform",
Electrophoresis (2008), in press.
|
| |
|
Wu, S.-L., Gu, Y., Hanlon, D., Meyer, J., Linder,
J., Hancock, W.S., and Karger, B.L., "Proteomic
Analysis of High-Grade Dysplastic Cervical Cells
Obtained From ThinPrep® Slides Using Laser
Capture Microdissection and Mass Spectrometry",
J Proteome Res., 6, 4256-68 (2007).
|
| |
|
Plavina, T., Wakeshull, E., Hancock, W.S., Hincapie,
M., "Combination of abundant protein depletion
and multi-lectin affinity chromatography (M-LAC)
for plasma protein biomarker discovery",
J. Proteome Res., 662-671 (2007). |
| |
|
Zheng, X., Baker, H., and Hancock, W. S., "Analysis
of the Low Molecular Weight Serum Peptidome
Using Ultrafiltration and a Hybrid Ion Trap-Fourier
Transform Mass Spectrometer", J. Chromatogr.,
1120, 173-184 (2006). |
| |
| Plymoth,
A., Yang, Z., Lofdahl, C. G., Ekberg-Jansson,
A., Dahlback, M., Fehniger, T. E., Marko-Varga,
G., Hancock, W. S., "Rapid Proteome Analysis
of Bronchoalveolar Lavage Samples of Lifelong
Smokers and Never-Smokers by Micro-Scale Liquid
Chromatography and Mass Spectrometry",
Clin. Chem., 52, 671-679 (2006). |
| |
| Wang, Y., Wu,
S. L. and Hancock, W. S., "Approaches to
the Study of N-linked Glycoproteins in Human
Plasma Using Lectin Affinity Chromatography
and Nano-HPLC Coupled to Electrospray Linear
Ion Trap-Fourier Transform Mass Spectrometry
(LTQ/FTMS)", Glycobiology, 16, 514-523
(2006). |
| |
|
Wang, Y., Wu, S. L. and Hancock, W. S., "Monitoring
of Glycoprotein Products in Cell Culture Lysates
Using Lectin Affinity Chromatography and Capillary-HPLC
Coupled to Electrospray Linear Ion Trap - Fourier
Transform Mass Spectrometry LTQ/FTMS)",
Biotechnology Progress, 22, 873-880 (2006). |
| |
| Zheng, X., Wu,
S. L., and Hancock, W. S., "Glycation of
Interferon-beta-1b and human serum albumin in
a lyophilized glucose formulation", Int.
J. Pharmaceutics, 322, 136-145 (2006). |
| |
| Yang,
Z., Harris, L. E., Palmer-Toy, D. E. and Hancock,
W. S., "Characterization of Multiple Glycoprotein
Biomarker Candidates in Serum from Breast Cancer
Patients Using Multi-lectin Affinity Chromatography
(M-LAC)", Clin. Chem., 52, 1897-1905
(2006). |
| |
| Wang,
Y., Dai, D., Sadee, W. and Hancock, W.S., "A
Pharmacoproteomics Study of the Cancer Cell
Line EKVX", Mol. Pharmaceutics,
5, 566-578 (2006). |
| |
| Zheng,
X., Baker, H., Hancock, W. S., Fawaz, F., McCaman,
M. and Pungor, E., "Proteomic Analysis
for the Assessment of Different Lots of Fetal
Bovine Serum as a Raw Material for Cell Culture",
Biotechnology Progress, 5, 1294-1300
(2006). |
| |
| Baker,
H., Patel, V., Molinolo, A.A., Shillitoe, E.J.,
Ensley, J.F., Yoo, G.H., Meneses-Garcia, A.,
Myers, J.N., El-Naggar, A.K., Silvio Gutkind,
J. and Hancock, W.S., "Proteome-Wide Analysis
of Head and Neck Squamous Cell Carcinomas Using
Laser-Capture Microdissection and Tandem Mass-Spectrometry",
J. Proteome Res., 41, 183-199
(2005). |
| |
| Yang,
Z.. and Hancock, W.S "Monitoring Glycosylation
Pattern Changes of Glycoproteins Using Multi-lectin
Affinity Columns", J. Chromatogr. A, 1070,
57-64 (2005). |
| |
|
Yang, Z., Hancock, W.S., Richmond-Chew, T.,
Bonilla, L., "A Study of Glycoproteins
in Human Serum and Plasma Reference Standards
(HUPO) using Multi-Lectin Affinity Chromatography
Coupled with RPLC-MS/MS", Proteomics,
13, 3353-66.(2005).
|
| |
| Wu,
S.-L, Kim, J.K., Hancock, W.S., and Karger,
B.L.,"Extended Range Proteomic Analysis
(ERPA): A New and Sensitive LC-MS Platform for
High Sequence Coverage of Complex Proteins with
Extensive Posttranslational Modifications -
Comprehensive Analysis of Beta-Casein and Epidermal
Growth Factor Receptor (EGFR)", J.Proteome
Res., 4(4), 1155-1170 (2005). |
| |
| Omenn
GS, States DJ, Adamski M, Blackwell TW, Menon
R, Hermjakob H, Apweiler R, Haab BB, Simpson
RJ, Eddes JS, Kapp EA, Moritz RL, Chan DW, Rai
AJ, Admon A, Aebersold R, Eng J, Hancock WS,
Hefta SA, Meyer H, Paik YK, Yoo JS, Ping P,
Pounds J, Adkins J, Qian X, Wang R, Wasinger
V, Wu CY, Zhao X, Zeng R, Archakov A, Tsugita
A, Beer I, Pandey A, Pisano M, Andrews P, Tammen
H, Speicher DW, Hanash SM, "Overview of
the HUPO Plasma Proteome Project: Results from
the pilot phase with 35 collaborating laboratories
and multiple analytical groups, generating a
core dataset of 3020 proteins and a publicly-available
database", Proteomics, 5(13),
3226-45 (2005). |
| |
| Ping
P, Vondriska T.M., Creighton C.J., Gandhi T,
Yang Z, Menon R, Kwon M.S., Cho S.Y., Drwal
G, Kellmann M, Peri S, Suresh S, Gronborg M,
Molina H, Chaerkady R, Rekha B, Shet A.S., Gerszten
R.E., Wu H, Raftery M, Wasinger V, Schulz-Knappe
P, Hanash S.M., Paik Y.K., Hancock W.S., States
D.J., Omenn G.S., Pandey A., "A functional
annotation of subproteomes in human plasma",
Proteomics. (13), 3506-19 (2005). |
| |
| Wang,
,Y.,Wu, S.-L. Hancock, W.S., Trala, R., Kessler,
M., Taylor, A.H., and Aon, J.C., "Proteomic
Profiling of Escherichia coli Proteins under
High Cell Density Fed-batch Cultivation with
Overexpression of Phosphogluconolactonase",
Biotech. Progr., 5, 1401-1411
(2005). |
| |
Hancock,
W.S., Wang,Y., Yang, Y., and Gruia-Gray, J,
"Multidimensional Liquid Chromatography:
A High-Resolution, Reroducible, and Versatile
Technique for Proteomic Analysis of Complex
Biological Samples, American Laboratory,
16-19 (2005).
|
|
|
|
|
|
