Tomas Rejtar 

Contact Info

Barnett Institute
341 Mugar Bldg.
360 Huntington Ave.
Tel: 617-373-4895
Fax: 617-373-2855
E-mail: t.rejtar AT neu.edu

Employment

Education

• Ph.D., Analytical Chemistry, Institute of Analytical Chemistry, Academy of Sciences and Masaryk University, Czech Republic, 1999
  
• M.S., Analytical Chemistry, Masaryk University, Czech Republic, 1995

Awards

• John N. Hatsopoulos Scholar, Barnett Institute, Boston, MA
• Award in Advanced Research 2000 and 2001, Barnett Institute, Boston, MA
• Dean’s List 1990-1995, Masaryk University, Czech Republic
• Dean’s Award 1993, Masaryk University, Czech Republic

Research Interests

• Bioanalytical Applications of Mass Spectrometry
  
  • Protein glycosylation
  • Analysis of intact proteins by CE and LC MS
  • Analysis of glycopeptides by MSⁿ
  • Analysis of glycans by MSⁿ
    • Positive and negative analysis of oligosaccharides by ESI ion trap
    • Automated data interpretation
    • Denovo glycan sequencing
  • Shotgun proteomics using LC MALDI-MS and LC ESI-MS
  • Coupling of MALDI MS to LC

   

• Analysis of Mass Spec Data
  
  • Analysis of proteomic data
    • Mapping of proteins to pathways
    • Automated protein annotation, GO
    • Quantitative proteomics
  • Computer applications in bioanalytical research
    • MS and MSn denoising
    • Signal processing
   
  • Standards in proteomics
  • High throughput analysis of mass spectrometry data
    • LIMS systems for proteomic data
      
• Other applications
  
  • High throughput analysis by MALDI-MS
    • Protein microarrays
    • MALDI imaging
      
  • Disease diagnostics, biomarkers
    

Other interests

• Computer programming Perl, VB, C/C++
• Bioinformatics software for biological applications (gene prediction, sequence alignment)
• Machine learning - neural networks, genetic algorithms
• Database applications - Oracle, MySQL
• Linux operating system
  

Links

Selected Presentations

• ASMS 2000 Long Beach, CA
  Poster: Vacuum Deposition Interface for Coupling Capillary Electrophoresis to Commercial MALDI/TOF MS Instrument
  T. Rejtar, P. Hu, J. Preisler, F. Foret and B.L. Karger
• ASMS 2001 Chicago, IL
  Poster: Performance of Vacuum Deposition Interface for Coupling of Capillary Electrophoresis to MALDI/TOF MS
  T. Rejtar, P. Hu, J. Preisler, F. Foret and B.L. Karger
• ASMS 2002 Orlando, FL
  Poster: Multicolumn High Resolution LC-MALDI MS System for High Throughput Proteomics
  T. Rejtar, A. R. Ivanov, H-S. Chen, V. Andreev, E. Moskovets and B. L. Karger
• ASMS 2003 Montreal, Quebeck
  Poster: Universal Deposition Device for Off-line Coupling of LC to MALDI MS and MS/MS
  T. Rejtar, H-S. Chen, E. Moskovets, L. Li, V. Andreev and B. L. Karger
• Methods in Protein Structure Analysis 2004, Seattle, WA
  Talk: New approaches to maximize the information content of proteomics samples using LC-MS/MS analysis
  T. Rejtar
• ASMS 2004 Nashville, TN
  Poster: Enhanced Processing of High-Resolution MS/MS spectra for Peptide Identification Using Database Searching
  T. Rejtar, H-S. Chen, V. Andreev, E. Moskovets and B. L. Karger
• Pittcon 2005, Orlando FL
  Talk: Extended Range Proteomic Analysis: A New and Sensitive Nano-LC-MS Approach for PTM Characterization and High Sequence Coverage
  T. Rejtar
• ASMS 2005 San Antonio, TX
  Poster: Rapid Quantitative Analysis Of Serum Glycoproteins By Lc-Maldi Ms Using 2-Khz Maldi Ms Instrument
  T. Rejtar, S. Pan, L. Li, E. Moskovets, H. Zhang, R. Aebersold and B. L. Karger
• ASMS 2006 Seattle, WA
  Talk: Characterization of membrane receptors at low levels using extended range proteomic analysis
  T. Rejtar
  
• First Warren Workshop on Glycoconjugate Analysis, UNH, Durham NH
  Talk: Automated annotation of glycopeptide MS/MS spectra using a linearized glycan structure database
  T.Rejtar
  
• ASMS 2007 Indianapolis, IN
  Poster: A New Strategy for Quantitative Proteomic Analysis of Organisms with Unsequenced Genomes
  T. Rejtar, M. Hincapie, J.T. Oldham, C. W.T. Lee-Parsons, J.G. Dy and B.L. Karger

Recent publications

• L. Li, Q. Li, L. Rohlin, U. Kim, K. Salmon, T. Rejtar, R.P. Gunsalus, B.L. Karger, J.G. Ferry Quantitative proteomic and microarray analysis of the archaeon Methanosarcina acetivorans grown with acetate versus methanol, PNAS accepted.
• F. Correia, A. D’Onofrio1, T. Rejtar, L. Li, B.L. Karger, K. Makarova, E.V. Koonin, K. Lewis Escherichia coli High-Persistence HipA Protein Contains a Kinase Domain Required for its Function, J. of Bacteriology, accepted.
• Andreev, V.; Li, L.; Li, Q.; Rejtar, T. ; Ferry, J.G; Karger, B.L. A New Algorithm for 15N/14N Quantitation with LC-ESI-MS Using an LTQ-FT Mass Spectrometer, J. of Proteome Research accepted.
• Li, Q.; Li, L.; Rejtar, T.; Lessner, D.J.; Karger, B.L.; Ferry, J.G.  Electron transport in the pathway of acetate conversion to methane in the marine archaeon Methanosarcina acetivorans.   
  J. of Bacteriology  (2006), 188(2), 702.
• Moskovets, E.; Preisler, J.; Chen, H. S.; Rejtar, T.; Andreev, V.; Karger, B. L.  High-Throughput Axial MALDI-TOF MS Using a 2-kHz Repetition Rate Laser.    Anal. Chem. (2006), 78(3), 912.
• Chen, H-S.; Rejtar, T.; Andreev, V.; Moskovets, E.; Karger, B.L.  Enhanced Characterization of Complex Proteomic Samples Using LC-MALDI MS/MS: Exclusion of Redundant Peptides from MS/MS Analysis in Replicate Runs Anal. Chem. (2005), 77(23), 7816.
• Chen, H-S.; Rejtar, T.; Andreev, V.; Moskovets, E.; Karger, B.L.  High-speed, high-resolution monolithic capillary LC-MALDI MS using an off-line continuous deposition interface for proteomic analysis.  Anal. Chem.  (2005), 77(8), 2323.
• Pashkova, A.; Chen, H-S; Rejtar, T.; Zang, X.; Giese, R.; Andreev, V.; Moskovets, E. and Karger, B.L. Coumarin Tags for Improved Analysis of Peptides by MALDI–TOF MS and MS/MS II. Alexa Fluor 350 Tag for Increased Peptide and Protein Identification by LC–MALDI–TOF/TOF MS, Anal. Chem. 2005; 77(7); 2085-2096.
• Li, Q.; Li, L.; Rejtar, T.; Karger, B.L.; Ferry, J.G. Proteome of Methanosarcina acetivorans Part I: An Expanded View of the Biology of the Cell.  J. of Proteome Research  (2005), 4(1), 112-128.
• Li, Q.; Li, L.; Rejtar, T.; Karger, B.L.; Ferry, J.G.    Proteome of Methanosarcina acetivorans Part II: Comparison of Protein Levels in Acetate- and Methanol-Grown Cells. J. of Proteome Research  (2005), 4(1),  129-135.

Last update Jan 2008