Tomas Rejtar

Research Assistant Professor

The Barnett Institute of Chemical and Biological Analysis
341 Mugar Hall
Northeastern University, 360 Huntington Avenue
Boston MA 02115

Tel: 617-373-4895
Fax: 617-373-2855
E-mail: t.rejtar AT neu.edu

Dr. Rejtar's research is in advanced methods for analysis of proteins. A major area is coupling high-resolution separations with mass spectrometry, either for profiling complex mixtures of proteins, or for detailed analysis of a single protein. For high-resolution separations, Dr. Rejtar has deep expertise in capillary electrophoresis methods, which are a powerful complement to microbore LC methods developed at the Institute. Tomas' technical expertise has also supported the development of new mass spectrometry techniques and hardware, and data processing methods (see publications)

Research Interests

Bioanalytical Applications of Mass Spectrometry
- Protein glycosylation
- Analysis of intact proteins by CE and LC MS
- Analysis of glycopeptides by MSⁿ
- Analysis of glycans by MSⁿ
  - Positive and negative analysis of oligosaccharides by ESI ion trap
  - Automated data interpretation
  - Denovo glycan sequencing
- Shotgun proteomics using LC MALDI-MS and LC ESI-MS
- Coupling of MALDI MS to LC
Analysis of Mass Spec Data
- Analysis of proteomic data
  - Mapping of proteins to pathways
  - Automated protein annotation, GO
  - Quantitative proteomics
- Computer applications in bioanalytical research
  - MS and MSn denoising
  - Signal processing
- Standards in proteomics
- High throughput analysis of mass spectrometry data
  - LIMS systems for proteomic data
Other applications
- High throughput analysis by MALDI-MS
  - Protein microarrays
  - MALDI imaging
- Disease diagnostics, biomarkers

Education

Ph.D., Analytical Chemistry, Institute of Analytical Chemistry, Academy of Sciences and Masaryk University, Czech Republic, 1999
M.S., Analytical Chemistry, Masaryk University, Czech Republic, 1995

Awards

John N. Hatsopoulos Scholar, Barnett Institute, Boston, MA
Award in Advanced Research 2000 and 2001, Barnett Institute, Boston, MA
Dean’s List 1990-1995, Masaryk University, Czech Republic
Dean’s Award 1993, Masaryk University, Czech Republic

Selected Presentations

ASMS 2000 Long Beach, CA
Poster: Vacuum Deposition Interface for Coupling Capillary Electrophoresis to Commercial MALDI/TOF MS Instrument
T. Rejtar, P. Hu, J. Preisler, F. Foret and B.L. Karger
ASMS 2001 Chicago, IL
Poster: Performance of Vacuum Deposition Interface for Coupling of Capillary Electrophoresis to MALDI/TOF MS
T. Rejtar, P. Hu, J. Preisler, F. Foret and B.L. Karger
ASMS 2002 Orlando, FL
Poster: Multicolumn High Resolution LC-MALDI MS System for High Throughput Proteomics
T. Rejtar, A. R. Ivanov, H-S. Chen, V. Andreev, E. Moskovets and B. L. Karger
ASMS 2003 Montreal, Quebeck
Poster: Universal Deposition Device for Off-line Coupling of LC to MALDI MS and MS/MS
T. Rejtar, H-S. Chen, E. Moskovets, L. Li, V. Andreev and B. L. Karger
Methods in Protein Structure Analysis 2004, Seattle, WA
Talk: New approaches to maximize the information content of proteomics samples using LC-MS/MS analysis
T. Rejtar
ASMS 2004 Nashville, TN
Poster: Enhanced Processing of High-Resolution MS/MS spectra for Peptide Identification Using Database Searching
T. Rejtar, H-S. Chen, V. Andreev, E. Moskovets and B. L. Karger
Pittcon 2005, Orlando FL
Talk: Extended Range Proteomic Analysis: A New and Sensitive Nano-LC-MS Approach for PTM Characterization and High Sequence Coverage
T. Rejtar
ASMS 2005 San Antonio, TX
Poster: Rapid Quantitative Analysis Of Serum Glycoproteins By Lc-Maldi Ms Using 2-Khz Maldi Ms Instrument
T. Rejtar, S. Pan, L. Li, E. Moskovets, H. Zhang, R. Aebersold and B. L. Karger
ASMS 2006 Seattle, WA
Talk: Characterization of membrane receptors at low levels using extended range proteomic analysis
T. Rejtar
First Warren Workshop on Glycoconjugate Analysis, UNH, Durham NH
Talk: Automated annotation of glycopeptide MS/MS spectra using a linearized glycan structure database
T.Rejtar
ASMS 2007 Indianapolis, IN
Poster: A New Strategy for Quantitative Proteomic Analysis of Organisms with Unsequenced Genomes
T. Rejtar, M. Hincapie, J.T. Oldham, C. W.T. Lee-Parsons, J.G. Dy and B.L. Karger

Recent publications

Thakur D, Rejtar T, Karger BL, Washburn NJ, Bosques CJ, Gunay NS, Shriver Z, Venkataraman G. Profiling the Glycoforms of the Intact alpha Subunit of Recombinant Human Chorionic Gonadotropin by High-Resolution Capillary Electrophoresis-Mass Spectrometry. Anal Chem. 2009 Oct 9. [Epub ahead of print] (pubmed)
Grunwald J, Rejtar T, Sawant R, Wang Z, Torchilin VP. TAT Peptide and Its Conjugates: Proteolytic Stability. Bioconjug Chem. 2009 Jul 14. [Epub ahead of print] (pubmed)
Monzo A, Rejtar T, Guttman A. Optimization of poly(GMA-co-EDMA) Monolithic Support for Trypsin Nanoreactor Fabrication. J Chromatogr Sci. Jul 2009; 47(6):467-72. (pubmed)
Baumgartner C, Rejtar T, Kullolli M, Akella LM, Karger BL. "SeMoP: A New Computational Strategy for the Unrestricted Search for Modified Peptides Using LC-MS/MS Data." J Proteome Res. 7(9):4199-4208. (2008) pubmed
Luo Q, Gu Y, Wu SL, Rejtar T, Karger BL. "Two-dimensional strong cation exchange/porous layer open tubular/mass spectrometry for ultratrace proteomic analysis using a 10 microm id poly(styrene- divinylbenzene) porous layer open tubular column with an on-line triphasic trapping column." Electrophoresis. 29(8):1604-11. (2008) pubmed
Lazarev AV, Rejtar T, Dai S, Karger BL. Centrifugal methods and devices for rapid in-gel digestion of proteins. Electrophoresis. Mar 2009; 30(6):966-73. (pubmed)
Zvonok, N., Yaddanapudi, S., Williams, J., Dai, S., Dong, K., Rejtar, T., Karger, B.L. and Makriyannis, A., " Comprehensive Proteomic Mass Spectrometric Chracterization of Human Cannabinoid CB2 Receptor", J. Proteome Research, 2068-2079, (2007).pubmed
Ren, J.M., Rejtar, T., Li, L. and Karger, B.L. "Automated N-Glycan Structure Annotation of Glycopeptides Using a Linearized Structure database (GlyDB)", J. of Proteome Research, 6, 3162-3173, (2007).
Andreev, V., Li, L., Cao, L., Gu, Y., Rejtar, T., Wu, S-L. And Karger, B.L. " A New Algorithm Using Cross-Assignment for Label-Free Quantitation with LC/LTQ-FT MS", J. of Proteome Res., 6, 2186-2194 (2007).
Li, L., Li, Q., Rohlin, L., Kim, U., Salmon, K., Rejtar, T., Gunsalus, R.P., Karger, B.L. and Ferry, J.G. "Quantitative proteomic and microarray analysis of the archaeon Methanosarcina acetivorans grown with acetate versus methanol", J. of Proteome Res, 6, 759-771, (2007).
L. Li, Q. Li, L. Rohlin, U. Kim, K. Salmon, T. Rejtar, R.P. Gunsalus, B.L. Karger, J.G. Ferry Quantitative proteomic and microarray analysis of the archaeon Methanosarcina acetivorans grown with acetate versus methanol, PNAS accepted.
F. Correia, A. D’Onofrio1, T. Rejtar, L. Li, B.L. Karger, K. Makarova, E.V. Koonin, K. Lewis Escherichia coli High-Persistence HipA Protein Contains a Kinase Domain Required for its Function, J. of Bacteriology, accepted.
Andreev, V.; Li, L.; Li, Q.; Rejtar, T. ; Ferry, J.G; Karger, B.L. A New Algorithm for 15N/14N Quantitation with LC-ESI-MS Using an LTQ-FT Mass Spectrometer, J. of Proteome Research accepted.
Li, Q.; Li, L.; Rejtar, T.; Lessner, D.J.; Karger, B.L.; Ferry, J.G. Electron transport in the pathway of acetate conversion to methane in the marine archaeon Methanosarcina acetivorans.
J. of Bacteriology (2006), 188(2), 702.
Moskovets, E.; Preisler, J.; Chen, H. S.; Rejtar, T.; Andreev, V.; Karger, B. L. High-Throughput Axial MALDI-TOF MS Using a 2-kHz Repetition Rate Laser. Anal. Chem. (2006), 78(3), 912.
Chen, H-S.; Rejtar, T.; Andreev, V.; Moskovets, E.; Karger, B.L. Enhanced Characterization of Complex Proteomic Samples Using LC-MALDI MS/MS: Exclusion of Redundant Peptides from MS/MS Analysis in Replicate Runs Anal. Chem. (2005), 77(23), 7816.
Chen, H-S.; Rejtar, T.; Andreev, V.; Moskovets, E.; Karger, B.L. High-speed, high-resolution monolithic capillary LC-MALDI MS using an off-line continuous deposition interface for proteomic analysis. Anal. Chem. (2005), 77(8), 2323.
Pashkova, A.; Chen, H-S; Rejtar, T.; Zang, X.; Giese, R.; Andreev, V.; Moskovets, E. and Karger, B.L. Coumarin Tags for Improved Analysis of Peptides by MALDI–TOF MS and MS/MS II. Alexa Fluor 350 Tag for Increased Peptide and Protein Identification by LC–MALDI–TOF/TOF MS, Anal. Chem. 2005; 77(7); 2085-2096.
Li, Q.; Li, L.; Rejtar, T.; Karger, B.L.; Ferry, J.G. Proteome of Methanosarcina acetivorans Part I: An Expanded View of the Biology of the Cell. J. of Proteome Research (2005), 4(1), 112-128.
Li, Q.; Li, L.; Rejtar, T.; Karger, B.L.; Ferry, J.G. Proteome of Methanosarcina acetivorans Part II: Comparison of Protein Levels in Acetate- and Methanol-Grown Cells. J. of Proteome Research (2005), 4(1), 129-135.