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Dr. Giese's research is in the detection and identification
of trace biomolecules such as genotoxins, DNA adducts,
steroids, and bio-active peptides by mass spectrometry. The
work combines analytical chemistry, organic chemistry, and
biochemistry to advance the reagents and techniques available
for sample preparation. Novel tags are developed for
derivatization to increase analyte response and selectivity. A
recent example is the development of a nucleotide exposure
mass profile test which discovers genotoxins in environmental
mixtures. In this test, a DNA nucleotide or strand is used as
bait to catch genotoxic chemicals, giving specific changes in
mass. Because accurate masses are determined, a connection can
be made to corresponding DNA adducts in biological samples
such as human blood lymphocytes. The opportunity to scale up
environmental samples provides a pathway to structural
characterization of unknown genotoxins. Below is a figure
demonstrating the diversity of DNA adducts that can be
detected simultaneously with high sensitivity and similar
response with this technology.
Environmental
Cancer Research Program Site
Prof. Giese's Research Group Site
Selected Publications (of 170
total)
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Yong,
L. C., Schulte, P. A., Kao, C-K., Giese, R. W., Boeniger,
M. F., Strauss, G. H.S., Petersen, M. R., Wiencke,
J. K., "DNA Adducts in Granulocytes of Hospital
Workers Exposed to Ethylene Oxide", Am. J.
Ind. Med., 50, 293-302.09 (2007).
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Shao, G., Kautz, R., Peng, S., Cui, G., Giese, R.
W., "Calibration by NMR for quantitative analysis:
p-Toluenesulfonic acid as a reference substance",
J. Chromatogr. A, 1138, 305-308 (2007).
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Murugaiah, V., Naim, A., Peng, S., Cui, G., Giese,
R. W., "4-(4'-Methyltetrafluorophenyl)-2,3,5,6-tetrafluorobenzyl
bromide: A new electrophoric derivatizing reagent",
J. Chromatogr113. A, 4, 338-340 (2006).
- Shimelis, O., Giese, R. W., "Nuclease P1 digestion/high-performance
liquid chromatography, a practical method
for DNA quantitation", J. Chromatogr.
A., 1117, 132-136 (2006).
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Kao, C-Y., Giese, R.W., Measurement of N7(2-Hydroxyethyl)guanine
in Human DNA by Gas Chromatography Electron Capture Mass
Spectrometry, Chem. Res. Toxicol, 18, 70-75 (2005).
- Pashkova,
A., Chen, H., Rejtar, T., Zang, X., Giese, R., Andreev,
V., Moskovets, E. Karger, Barry L. "Coumarin Tags for
Improved Analysis of Peptides by MALDI-TOF MS and MS/MS.
II. Alexa Fluor 350 Tag for Increased Peptide and Protein
Identification by LC-MALDI-TOF/TOF MS", Anal. Chem.,
4(1), 129-135 (2005).
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