|
Education
1965
Ph.D., MIT
1961
B.A., Wesleyan University
Research
Interests
The
central focus of our research is in the area of mass spectrometry
and its applications to organic analysis. Our efforts involve
the investigation of both fundamental and applied problems.
We have established a strong reputation for the development
of techniques that involve the interfacing of separation
methods - gas chromatography, liquid chromatography, and
capillary zone electrophoresis - with mass spectrometry.
Optimization of these sample delivery systems has enabled
us to advance the state of the art for trace level analysis
of biological samples. Much of our applied work is being
done in collaboration with colleagues from the biomedical
community in the Greater Boston area and beyond.
Selected Publications
- Williams J, Wood J, Pandarinathan L,
Karanian DA, Bahr BA, Vouros P, Makriyannis A. "Quantitative
method for the profiling of the endocannabinoid metabolome
by LC-atmospheric pressure chemical ionization-MS."
Anal Chem. ;79(15):5582-93. 2007 Aug 1; Epub 2007
Jun 29 pubmed
- Xiong W, Glick J, Lin Y, Vouros P. "Separation
and sequencing of isomeric oligonucleotide adducts using
monolithic columns by ion-pair reversed-phase nano-HPLC
coupled to ion trap mass spectrometry."
Anal Chem. 2007 Jul 15;79(14):5312-21. Epub 2007 Jun
19 pubmed
- Black TM, Andrews CL, Kilili G, Ivan M,
Tsichlis PN, Vouros P. Characterization of
phosphorylation sites on Tpl2 using IMAC enrichment and a
linear ion trap mass spectrometer. J Proteome Res.
2007 Jun;6(6):2269-76. Epub 2007 May 2 pubmed
-
Liao, Q., Chiu, N. H. L., Shen, C., and
Vouros, P., “Investigation of the Enzymatic
Behavior of Benzonase /Alkaline Phosphatase
in the Digestion of DNA and DNA Adducts
by ESI-LC/MS”, Anal. Chem.,
79, 1907-1917 (2007). pubmed
- Williams J, Pandarinathan L, Wood J, Vouros
P, Makriyannis A. Endocannabinoid metabolomics: a novel
liquid chromatography-mass spectrometry reagent for fatty
acid analysis. AAPS J. 2006 Oct 20;8(4):E655-660. DOI: 10.1208/aapsj080474.
free
full text
- Reddy GS, Robinson M, Wang G, Palmore GT,
Gennaro L, Vouros P, De Clercq P, Vandewalle M, Young W,
Ling S, Verstuyf A, Bouillon R. Removal of C-ring from
the CD-ring skeleton of 1alpha,25-dihydroxyvitamin D3 does
not alter its target tissue metabolism significantly.
Arch Biochem Biophys. 2007 Apr 15;460(2):254-61. Epub 2006
Nov 21 pubmed
-
- Levin DS, Vouros P, Miller RA, Nazarov EG. Using
a nanoelectrospray-differential mobility spectrometer-mass
spectrometer system for the analysis of oligosaccharides
with solvent selected control over ESI aggregate ion
formation. J Am Soc Mass Spectrom. 2007
Mar;18(3):502-11. Epub 2006 Nov 30. pubmed
- Ricicki EM, Luo W, Fan W, Zhao LP, Zarbl H,
Vouros P. Quantification of
N-(deoxyguanosin-8-yl)-4-aminobiphenyl adducts in human
lymphoblastoid TK6 cells dosed with
N-hydroxy-4-acetylaminobiphenyl and their relationship to
mutation, toxicity, and gene expression profiling.
Anal Chem. 2006 Sep 15;78(18):6422-32 pubmed
- Levin DS, Miller RA, Nazarov EG, Vouros
P. Rapid separation and quantitative analysis of
peptides using a new nanoelectrospray- differential
mobility spectrometer-mass spectrometer system. Anal
Chem. 2006 Aug 1;78(15):5443-52 pubmed
-
Andrews, C.L., Li, F., Yu, C.P., Yang, E.,
and Vouros, P., “Incorporation of a
Nanosplitter Interface into an LC-RD-MS
System to Facilitate Drug Metabolism Studies”,
Journal of Mass Spectrom. 41,
43-49 (2006). pubmed
- Levin,
D.S., Vouros, P., Miller, R.A., Nazarov,
E.G., Morris, J.C., “Characterization
of gas phase interactions utilizing a novel
nanoelectrospray ionization - differential
mobility - mass spectrometer (nanoESI-DMS-MS)
system”, Anal. Chem., 78,
96-106 (2006). pubmed
Argoti D, Liang L, Conteh A, Chen L, Bershas D, Yu CP,
Vouros P, Yang E. Cyanide trapping of iminium ion
reactive intermediates followed by detection and structure
identification using liquid chromatography-tandem mass
spectrometry (LC-MS/MS). Chem Res Toxicol. 2005
Oct;18(10):1537-44. pubmed
-
Ricicki, E.M., Andrews, C.L., Schiavo, S.,
and Vouros, P., “Analysis of DNA Adducts”,
in Encyclopedia of Mass Spectrometry, Volume
3, Biological Applications Part B: Carbohydrates,
Nucleic Acids and other Biological Compounds,
Series editors: Caprioli, R. and Gross,
M., Elsevier Ltd., Oxford, 2006, pp. 61-85.
- Simionato,
A.V.C. , de Souza, G.D., Rodrigues-Filho,E.,
Glick, J., Vouros, P., and Carrilho, E.,
“Tandem mass spectrometry of coprogen
and deferoxamine hydroxamic siderophores”,
Rapid Commun. Mass Spectrom., 20,
193-199 (2006). pubmed
Flarakos J, Morand KL, Vouros P. High-throughput
solution-based medicinal library screening against human
serum albumin. Anal Chem. 2005 Mar
1;77(5):1345-53. pubmed
- Andrews CL, Yu CP, Yang E, Vouros P. Improved
liquid chromatography-mass spectrometry performance in
quantitative analysis using a nanosplitter interface.
J Chromatogr A. 2004 Oct 22;1053(1-2):151-9. pubmed
-
|
